Ihis study was purposed to observe the bone remodelling in implant interface. IMZ implants with 3.3㎜ in diameter and 8㎜ in length were implanted in rabbit´s tibia, 50㎎/㎏ Alizalin red and 20㎎/㎏ oxytetracyclin were injected intramus cullary 3 week and 1 weeks before sacrification. Experimental animal were sacrificed in 6,8,10,12 weeks after implantarion.
Bones with implants were removed after sacrification. Removed bones were fixed with 10% neutral formalin, dehydrared, and embedded with Spurr low viscosity resin. After the specimen were cut by 300㎛ with slow diamond wheel saw, these were ground up to 150㎛ using sand paper and polishing film. Ground specimen were stained with 1% toluidine blue staining solution. The stained ground specimen were examined with optical microscope for bone remodelling of implant interface. Laser Scanning Microsoope was used for checking the fluorecent labelling to the new bone. The obtained results were as follows.
1. In toluidin blue staining of 6 weeks, unilateral, irregular trabecular formation with irregular arranged osteocytes was
attached to the implant interface. Tn LSM features, AZ and TC labelled bone around implant were seen.
2. In toluidin blue staining of 8 weeks, unilateral, thicker cortical bone with Harversian canals in the upper portion com pared with that of 6 weeks, and compact bone surrounding the lower portion of implant were seen. In LSM features, AZ and TC labelled bone adjacent to the upper portion of implant were prominantly seen.
3. In toluidin blue staining of 10 weeks, cortical bone was grown into spongious bone. In LSM features; AZ and TC labelled bone were decreased, but AZ labelled bone adjacent to the implant interface were partially seen, and in the lower portion, weak AZ labelled bone was seen.
4. In toluidin blue staining of 12 weeks, osteocytes and microtubules were seen perpendiculary to well calcified bone. Which are containing Harversin canals attached to the implant interface. In LSM features, AZ and TC labelled bones which are adjacent to the implant interface were weakly seen.
From above results, it was suggested that there was the most active new bone formation between 5 to 8 weeks, while weak between 8 to 12 weeks, and at 12 weeks there were complete osseointegration between implant and well calcified bone tissue containing increased osteocytes and Harversian canals.
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